Biosci Rep
.
2020 Apr 30;40(4):BSR20192257.
doi: 10.1042/BSR20192257.
miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5
Jun Yao 1, Ruoyu Gao 1, Minghan Luo 1, Defeng Li 1, Liliangzi Guo 1, Zichao Yu 1, Feng Xiong 1, Cheng Wei 1, Benhua Wu 1, Zhenglei Xu 1, Dingguo Zhang 1, Jianyao Wang 2, Lisheng Wang 1
Affiliations expand
PMID: 32211804
PMCID: PMC7138906
DOI: 10.1042/BSR20192257
Retraction in
Retraction: miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5.
[No authors listed]Biosci Rep. 2024 Nov 27;44(11):BSR-2019-2257_RET. doi: 10.1042/BSR-2019-2257_RET.PMID: 39501751 Free PMC article. No abstract available.
Expression of concern in
Expression of Concern: miR-802 participates in the inflammatory process of inflammatory bowel disease by suppressing SOCS5.
[No authors listed]Biosci Rep. 2023 Jun 28;43(6):BSR-2019-2257_EOC. doi: 10.1042/BSR-2019-2257_EOC.PMID: 37272744 Free PMC article. No abstract available.
Abstract
The present study aims to reveal the detailed molecular mechanism of microRNA (miR)-802 in the progression of inflammatory bowel disease (IBD). IBD tissues were obtained from IBD patients, followed by CD4+ cells isolation. Then, qRT-PCR and ELISA were used to detect the expression of miR-802, suppressor of cytokine signaling 5 (SOCS5), interleukin (IL)-17A and tumor necrosis factor (TNF)-α. Transfection of miR-802 mimics and miR-802 inhibitor in CD4+ cells was detected by Western blot. TargetScan and luciferase reporter assay were used to detect the relationship between SOCS5 and miR-802. Finally, colitis mice model was established to verify whether miR-802 inhibitor was involved in the protective effect of colonic mucosa. The miR-802 was highly expressed in inflamed mucosa and PBMC cells of IBD. The highest expression of miR-802 was observed in CD4+ T cells based on different immune cell subsets analysis. SOCS5 was the target gene of miR-802. The mice model experiments showed that blockade of miR-802 could alleviate mice colitis. Our study suggests that up-regulation of miR-802 plays an important role in inflammatory process of IBD via targeting SOCS5. Moreover, the differentiation of Th17 and secretion of TNF-α in IBD could be stimulated by miR-802.
Keywords: SOCS5; TNF-α; Th17; inflammatory bowel disease; miR-802; mice colitis model.
参考链接：https://pubpeer.com/publications/B82BF8707890060BBA7966CCCDDD26
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