前面我们在单细胞天地公众号介绍过一下新工具新算法：《单细胞视角|五大策略助力细胞注释、T细胞免疫治疗新潜力、储存样本再利用新突破、高效单细胞分析工具CSI-GEP...》，其中有一个简介 吸引了我的注意：五大高效策略助力细胞类型注释。来看下！
网页介绍：https://pluto.bio/resources/Learning%20Series/annotating-clusters-in-scrnaseq
这个网页对当前的单细胞亚群注释做了一些详细介绍，并总结了五大策略：
第一种策略：利用现有的基因集和参考数据库 第二种策略：借助人工智能（AI）工具 第三种策略：应用机器学习方法 第四种策略：使用算法方法或专用软件 第五种策略：结合领域知识进行手动注释 综合策略：结合多种方法，确保注释准确性
可以看到当前的热门人工智能也列入其中，并且页面末尾给出一个非常全面的注释工具列表：
| Name | Category | URL |
|---|---|---|
| Human Cell Atlas (HCA) | Database | https://www.humancellatlas.org/ |
| PanglaoDB | Database | https://panglaodb.se/ |
| Allen Brain Atlas | Database | https://portal.brain-map.org/cell-types |
| MapMyCells | Other algorithm / tool | https://portal.brain-map.org/atlases-and-data/bkp/mapmycells |
| scGPT | AI / ML method | https://github.com/bowang-lab/scGPT |
| CellTypist | AI / ML method | https://www.celltypist.org/ |
| scMatch | AI / ML method | https://github.com/asrhou/scMatch |
| scCatch | Other algorithm / tool | https://github.com/ZJUFanLab/scCATCH |
| Seurat | Other algorithm / tool | https://satijalab.org/seurat/ |
| SingleR | Other algorithm / tool | https://bioconductor.org/packages/release/bioc/html/SingleR.html |
| SCINA | Other algorithm / tool | https://dbai.biohpc.swmed.edu/scina/ |
| Garnett | AI / ML method | https://cole-trapnell-lab.github.io/garnett/ |
| CellAssign | AI / ML method | https://github.com/Irrationone/cellassign |
| scPred | AI / ML method | https://github.com/powellgenomicslab/scPred |
| singleCellNet | AI / ML method | https://github.com/CahanLab/singleCellNet |
| CHETAH | AI / ML method | https://github.com/jdekanter/CHETAH |
| OnClass | AI / ML method | https://onclass.readthedocs.io/en/stable/ |
| scClassify | AI / ML method | https://bioconductor.org/packages/release/bioc/html/scClassify.html |
| scNym | AI / ML method | https://github.com/calico/scnym |
| ACTINN | AI / ML method | https://github.com/mafeiyang/ACTINN |
| scReClassify | AI / ML method | https://www.bioconductor.org/packages/release/bioc/html/scReClassify.html |
| Moana | AI / ML method | https://github.com/yanailab/moana |
| CaSTLe | AI / ML method | https://github.com/yuvallb/CaSTLe |
| CellMeSH | AI / ML method | https://github.com/shunfumao/cellmesh |
| cellHarmony | Other algorithm / tool | http://www.altanalyze.org/cellHarmony/ |
| Cell BLAST | AI / ML method | https://cblast.gao-lab.org/ |
| CIPR | Other algorithm / tool | https://aekiz.shinyapps.io/CIPR/ |
| Capybara | AI / ML method | https://github.com/morris-lab/Capybara |
| gCAnno | AI / ML method | https://github.com/xjtu-omics/gCAnno |
| SCSA | Other algorithm / tool | https://github.com/bioinfo-ibms-pumc/SCSA |
| scTyper | Other algorithm / tool | https://github.com/omicsCore/scTyper |
| NS-Forest | AI / ML method | https://github.com/JCVenterInstitute/NSForest |
| MarkerCapsule | AI / ML method | https://github.com/sumantaray/MarkerCapsule |
| HieRFIT | AI / ML method | https://github.com/yasinkaymaz/HieRFIT |
| scPretrain | AI / ML method | https://github.com/ruiyi-zhang/scPretrain |
| scSorter | AI / ML method | https://cran.r-project.org/web/packages/scSorter/index.html |
| SciBet | AI / ML method | http://scibet.cancer-pku.cn/ |
| MARS | AI / ML method | https://github.com/snap-stanford/mars |
| scClassifR | AI / ML method | https://github.com/grisslab/scClassifR |
| clustifyr | AI / ML method | https://github.com/rnabioco/clustifyR |
| Superscan | AI / ML method | https://github.com/cshasha/superscan |
| scDetect | AI / ML method | https://github.com/IVDgenomicslab/scDetect/ |
| ItClust | AI / ML method | https://github.com/jianhuupenn/ItClust |
| sigGCN | AI / ML method | https://github.com/NabaviLab/sigGCN |
| CALLR | AI / ML method | https://github.com/MathSZhang/CALLR |
| scANVI | AI / ML method | https://github.com/scverse/scvi-tools |
| CellO | AI / ML method | https://github.com/deweylab/CellO |
| Azimuth | Other algorithm / tool | https://azimuth.hubmapconsortium.org/ |
| scDeepSort | AI / ML method | https://github.com/ZJUFanLab/scDeepSort |
| ELeFHAnt | AI / ML method | https://github.com/praneet1988/ELeFHAnt |
| scMRA | AI / ML method | https://github.com/ddb-qiwang/scMRA-torch |
| scMRMA | AI / ML method | https://github.com/JiaLiVUMC/scMRMA |
| MACA | Other algorithm / tool | https://github.com/ImXman/MACA |
| GraphCS | Other algorithm / tool | https://github.com/biomed-AI/GraphCS |
| scAnnotatR | Other algorithm / tool | https://github.com/grisslab/scAnnotatR |
| ScType | AI / ML method | https://github.com/IanevskiAleksandr/sc-type/ |
| MarkerCount | Other algorithm / tool | https://github.com/combio-dku/MarkerCount/tree/master |
| scLearn | AI / ML method | https://github.com/bm2-lab/scLearn |
| scID | Other algorithm / tool | https://batadalab.github.io/scID/ |
| CellFishing.jl | Other algorithm / tool | https://github.com/bicycle1885/CellFishing.jl |
| SELINA | AI / ML method | https://github.com/SELINA-team/ |
| Census | AI / ML method | https://github.com/sjdlabgroup/Census |
| scGAA | AI / ML method | https://github.com/kongtianci/scGAA |
| TOSICA | AI / ML method | https://github.com/JackieHanLab/TOSICA |
| CASSIA | AI / ML method | https://www.cassiacell.com/ |
