高效硝酸盐还原菌（Bacillus velezensis M3-1）耦合强化厌氧氨氧化脱氮
Enhanced nitrogen removal of the anaerobic ammonia oxidation process by coupling with an efficient nitrate reducing bacterium (Bacillus velezensis M3-1)
Wanlian Yuan, Dongmin Yang, Xupo Zhang, Cancan Jiang, Danhua Wang, Jialiang Zuo, Shengjun Xu*, Xuliang Zhuang*
https://doi.org/10.1016/j.jes.2023.03.041
摘要
从狐尾藻人工湿地沉积物中分离得到的Bacillus velezensis M3-1菌株能够高效地将NO3−-N转化为NO2−-N，且对碳源添加量的要求不是很严格。这项工作首次表明，使用厌氧氨氧化和贝莱斯芽孢杆菌 M3-1微生物协同脱氮的可行性。在本研究中，在厌氧反应器中验证了M3-1将厌氧氨氧化产生的NO3−-N转化为NO2−-N的可能性。在不同C/N条件下，研究了M3-1与反硝化细菌耦合系统中NO3−-N的还原能力，发现M3-1对碳源的利用优于其他反硝化细菌。通过调节NH4+-N与NO2−-N的比例，发现NO3−-N在M3-1的作用下转化所得的NO2−-N参与了原厌氧氨氧化反应，在C/N = 2:1时，耦合系统的脱氮效率（NRE）比对照组厌氧氨氧化系统提高了12.1%。本研究表明，菌株M3-1对硝酸盐的还原速率足以去除厌氧氨氧化工艺中产生的硝酸盐，这将有助于提高废水的脱氮效果。
亮点
本研究证实了短程反硝化菌株Bacillus velezensis M3-1能够积累NO2−-N以强化厌氧氨氧化体系，且该强化过程在低C/N废水环境中成立。
Abstract
Bacillus velezensis M3-1 strain isolated from the sediment of Myriophyllum aquatium constructed wetlands was found to efficiently convert NO3−-N to NO2−-N, and the requirements for carbon source addition were not very rigorous. This work demonstrates, for the first time, the feasibility of using the synergy of anammox and Bacillus velezensis M3-1 microorganisms for nitrogen removal. In this study, the possibility of M3-1 that converted NO3−-N produced by anammox to NO2−-N was verified in an anaerobic reactor. The NO3−-N reduction ability of M3-1 and denitrifying bacteria in coupling system was investigated under different C/N conditions, and it was found that M3-1 used carbon sources preferentially over denitrifying bacteria. By adjusting the ratio of NH4+-N to NO2−-N, it was found that the NO2−-N converted from NO3−-N by M3-1 participated in the original anammox. The nitrogen removal efficacy (NRE) of the coupled system was increased by 12.1%, compared to the control group anammox system at C/N = 2:1. Functional gene indicated that it might be a nitrate reducing bacterium.This study shows that the nitrate reduction rate achieved by the Bacillus velezensis M3-1 can be high enough for removing nitrate produced by anammox process, which would enable improve nitrogen removal from wastewater.
作者简介
第一作者
袁万联硕士于2023年在中国科学技术大学获得硕士学位，期间由中国科学院生态环境研究中心、中国科学院环境生物技术重点实验室联合培养。
通讯作者
庄绪亮研究员于2001年在中国科学院生态环境研究中心获得理学博士学位。现任中国科学院青藏高原研究所党委书记、副所长（主持工作）。主要从事环境生物地球化学循环过程及机理研究，并开展流域多介质复合污染控制技术研发。在Global Change Biology、Environmental Science & Technology、Water Research等国际一流期刊发表SCI论文160余篇，授权国家发明专利30余项。
徐圣君博士于2014年在中国科学院生态环境研究中心获得工学博士学位。现任中国科学院生态环境研究中心副研究员。主要从事流域综合治理与生物调控技术研发与工程应用工作。在环境领域发表SCI论文20余篇，申请发明专利17项。
原文链接
https://www.sciencedirect.com/science/article/pii/S1001074223001559
引用格式
Wanlian Yuan, Dongmin Yang, Xupo Zhang, Cancan Jiang, Danhua Wang, Jialiang Zuo, Shengjun Xu, Xuliang Zhuang, 2024. Enhanced nitrogen removal of the anaerobic ammonia oxidation process by coupling with an efficient nitrate reducing bacterium (Bacillus velezensis M3-1). J. Environ. Sci. 146, 3-14.
